The aim of this investigation was to investigate the impacts of humic acid (H) and Salicylic acid foliar application (SA) on growth (plant height, number of branches/plant, number of leaves, fresh and dry weight of /herb and volatile oil percentage and it content of Basil (Ocimum basilicum L.) seedlings were investigated during period from August 2024 to January 2025 in Misurata city, Libya.Four levels of humic acid including 0, 2, 4 and 6 ml/L in main plots. Four levels of Salicylic acid (SA) were assigned in sub-plots at (0, 100, 200 and 300 ppm) as well as their interactions. The results demonstrated that the use of humic and salicylic acids significantly improved the growth parameters, and oil extraction. Regarding this concern, the highest results were recorded through the addition of a high level of humic acid (6 ml/L) along with foliar spray of Salicylic acid (300 ppm) in comparison to the control during period growing. The volatile oil's GC-MS analysis revealed that the usage of biostimulant applications also had an impact on the main ingredients. When plants were sprayed with 300 ppm of salicylic acid and a higher standard humic acid (6 ml/L), the proportions of key components were generally greater than those of untreated plants.