This investigation was carried out during 2010 and 2021 seasons on Early Sweet and Thompson Seedless grapevines cvs., cultivated in sandy soil located at Malawi district - El-Minia Governorate, Egypt. The two examined cvs. were irrigated by using drip irrigation system. This study was focused on the response of Early Sweet and Thompson Seedless cvs. to inoculation with Arbuscular Mycrrohiza Fungi (AMF), Azospirillum bacteria and Azotobacter bacteria individually or in combination. The obtained shows that inoculated the Early Sweet and Thompson Seedless were very effective in improving the nutritional status, productivity and berry physical and chemical properties. The combined inoculations with any two micro-organisms were more effective than the individual inoculations. However, the best results were obtained when the vines received the three micro-organisms in combination. Then, it is strongly recommended to inoculating the Early Sweet and Thompson Seedless cvs. grown in sandy soil with AMF, Azospirillum and Azotobacter in combination three times yearly, to enhancing the nutritional status, productivity and improving berries physical and chemical properties.