This study was executed during two growing seasons in 2021 and 2022 at a private vineyard located in Al Hawartah, East El-Nile, Minia Governorate. The study investigated the impact of foliar spraying with seaweed extract at concentrations of (0.05%, 0.1% & 0.2%) and three amino acids (methionine, tryptophan, and cystine) at concentrations of (50, 100 & 200 ppm). These treatments were administered three times during the vegetative growth stage, after set, and at one-month intervals. The study aimed to assess the effects on vegetative growth parameters, leaves pigments and leaves nutrient status in ten-year-old "Superior seedless" grapevines. The 30 vines selected for the experiment have as similar vigor as feasible and planted in clay soil. Result of the present work suggested that spraying with seaweed extract was more effective than the amino acids. Combining applications enhanced the parameters more than utilizing each material alone especially at amino acids (200 ppm) + seaweed extract (0.2%), which scored the highest values of primary shoot length, number of leaves per plant, leaf area, pruning wood weight, cane thickness, leaves (chlorophyll, carotenoid content, N, P, K, Fe, Mn and Zn concentration). Lower concentrations had no effect. Based on the current work's findings, it is recommended for economic reasons to spray Superior grapevines with amino acids (100 ppm) + seaweed extract (0.1%), which gave the best vegetative growth and good nutrient content of leaves.