The present investigation was conducted during two successive seasons 2018 and 2019 on own rooted 17 years old ‘Superior Seedless’ grapevine grown at Matay district El-Minia Governorate. This study aimed to investigate the response of growth and fruiting of Superior Seedless grapevine cv. to spraying seaweed extract or/and chitosan. It is clear from the obtained results that, using both examined materials (seaweed extract and chitosan) in combination was more effective than using each one alone. Non-significant differences between the two highest concentrations of both examined compounds (seaweed or/and chitosan) were observed, during the two experimental seasons. It is may be practical recommended to spray ‘Superior Seedless’ grapevines grown in clay soil conditions with seaweed extract at 0.2% + chitosan at 0.2% three times yearly in order to improve vegetative growth parameters, vines mineral status and productivity of ‘Superiors Seedless’ grapevines.