This research was carried out over two consecutive seasons (2022 and 2023) to study the impact of jasmonic acid at concentration of (15, 30 and 45 ppm) and naphthalene acetic acid at concentration of (50, 100 and 150 ppm) as well as their interactions at different dosage on 10-year-old Flame seedless grapevines in clay soil at a private farm in Talla village, Minia Governorate. The Results showed that applications of jasmonic acid + naphthalin acetic acid were effective in contrast to the single application and the control for improving yield, cluster parameters and physio-chemical quality of Flame Seedless berries and the best treatment was 45 ppm jasmonic acid + 150 ppm naphthalin acetic acid followed by 30 ppm jasmonic acid + 100 ppm naphthalin acetic acid, with no discernible distinction between the two and considered the middile concentration was the best, which gave the promising results in this respect.