A field investigation was conducted during 2020 and 2021 seasons on Flame Seedless and Red Globe grapevines cvs., grown in sandy soil located at Malawi district - El-Minia Governorate, Egypt. The examined cvs were irrigated by using drip irrigation system and planted at 2 X 3 meters apart. This investigation was focused on the response of Flame Seedless and Red Globe cvs. to inoculation with Arbuscular Mycrrohiza Fungi (AMF), Azospirillum bacteria and Azotobacter bacteria individually or in combination. The obtained results show that, when using each micro-organism individually, the effect of AMF on yield and its component as well as berries physical and chemical properties was superior to using Azospirillum or Azotobacter each one individually. The combined inoculation shows more effective than the individual inoculations. The vines of both cultivar inoculating with the three micro-organisms in combination (AMF + Azospirillum + Azotobacter) produced the best yield and berry physical and chemical properties.