Nano fertilization utilizing zinc and iron oxide nanoparticles is an effective approach to enhance and improve the nutritional quality of the plant's edible portion. The aim was to compared the efficiency of traditional and nano fertilization in form of zinc and iron on yield and quality of Flame seedless grapevines. The treatments were distributed using a completely randomized design, comprising seven treatments, administered by foliar sprays three times. Results suggested that foliar application of nano Zn (20 ppm) in conjunction with Fe (40 ppm), followed by a lower dosage of nano-oxide fertilizer, which did not exhibit significant differences (P≤0.05) between them, positively influences cluster characteristics, yield, and the physico-chemical quality of the berries under investigation. From the data, it could conclude that foliar application of nano Zn (10 ppm) in conjunction with Fe (20 ppm) serves as an alternative to conventional fertilizers (EDTA) for enhancing the quality and productivity of Flame seedless grapevines.