High temperature regions cause major issues for grapes in terms of yield quality characteristics. Therefore, meeting consumer and market demands necessitates that growers work to improve berry quality. A number of technical advances have recently been suggested as ways to lessen the reliance on agrochemicals, which would have a positive impact on both output and sustainability. Using eco-friendly organic goods like "Biostimulants" is one of the greatest ways to boost crop output. Both “Stimulant and Acadian” are examples of commercially available products with biostimulant properties. Enhancing plant growth, yield, and product quality are crucial functions of these biostimulants. This study was carried out through the seasons of 2019 and 2020 at a private vineyard situated west of Bani Mazar Center in Minia Governorate. Roomy Red grapevines (16 years), a cultivar that used. This research aims to compare the efficacy of two commercially available products—Stimulant and Acadian—when tested with different concentrations (0.25, 0.5 and 1 g/L) on yield and berries physio-chemical quality Roomy Red grapevines compared to the untreated vines. The application of both products was associated with the enhancement of berry yield and physio-chemical properties. In terms of yield, cluster characteristics, berry chemical and physical parameters, reduction of shot berries/clusters, and total acidity, Acadian was more successfully than Stimulant, particularly at 1 g/L and then 0.5 g/L without a discernible change between them. Therefore, it is feasible to suggest spraying 0.5 g/L from Acadian for economical reasons. They provided the highest-quality berries at a manageable output.