Germplasm conservation is considered the most valuable way to preserve endangered genotypes and commercial species. Besides, it is an important method for improving plant breeding programs through either in situ or ex situ preservation techniques. The most suitable ex situ conservation technique is in vitro culture under controlled conditions. This investigation is to evaluate the response of the Egyptian cultivar “Manfaloty” pomegranate germplasm to preservation through encapsulation technique using nodal segments for short and medium-term storage periods. Encapsulation was carried out by using sodium alginate at 2% and 3%. Both 2% and 3% Na-alginate treatments were cultured on MS media supplemented with Abscisic acid at 0.1, 0.2 and 0.3 mg/l and mannitol at 1.0, 2.0 and 3.0 g/l, in addition to free MS as a control treatment. Regrowth was evaluated as the percentage of capsules viability, growth and size increasing. Encapsulation using sodium alginate at 3 % gave the best results compared with 2%. Sodium alginate with free MS medium was the superior treatment for surviving and regrowth rates of preserved beads (100%), in compared to abscisic acid and mannitol. capsules that cultured with ABA recorded the highest survival percentage compared with the other capsules that cultured with Mannitol, the survival percentages were (83%, 66.6% and 50%) with ABA at 0.1, 0.2 and 0.3 mg/L, respectively and (50%, 25% and 16.6%) with Mannitol at 1.0, 2.0 and 3.0 mg/L, respectively. Adding ABA and mannitol to medium slowing and keeping the encapsulated nodal segments at low rates of growth led to prolonging the preservation periods at low concentrations. However, it suppressed the regrowth percent at high concentrations, which might be due to their inhibitory and osmotic influences. Moreover, increasing of storage duration at 7°C, the regrowth and survival percentages gradually declined; the survival percentages of encapsulated nodal segments and regrowth to survival of were 40.0 and 45.0%, respectively, after 12 weeks. This decline in growth rate of encapsulated nodal segments stored at low temperatures may be due to the inhibited respiration of plant tissues caused by the alginate cover.