Recently, the demand for grafted grape on different rootstocks has been increased because of its benefit to combat the soil problems and enhancing productivity. Also, rootstocks are selected primarily based on their ability to withstand range of typical soil conditions, especially those that are important to maintaining vine vigor in the face of significant abiotic stresses. The current study was carried out during three years (2019, 2020, and 2021) to the effect of Freedom, Salt Creek, and SO4 rootstocks of H4 strain and Sultana grapevines on vegetative growth and biochemical parameters. The experiment was arranged in a randomized complete block design of eight treatments with three replicates with four trees each. The results indicate that H4 strain and Sultana grapevines grafted on the rootstock of Freedom enhanced the vegetative growth and biochemical parameters followed by grafted on Salt Creek and SO4 rootstock, respectively. Contrary, H4 strain and Sultana grapevines un-grafted (own rooted) recorded the least values of vegetative growth and biochemical parameters. In addition, the H4 strain grafted on studied rootstocks had superiority to grafted Sultana grapevines. The best results were achieved with the grafted grapevines on Freedom rootstock. Therefore, it can be recommended that graft H4 strain and Sultana grapevines on Freedom rootstock in clay soils under flood-irrigated system.