Vegetative growth aspects and leaves nutritional status of 8-year-old Early Sweet grapevines grown in clay soil in response to applying various concentrations of royal jelly (100, 200 and 400 ppm) and/or glutathione at (250, 500 and 1000 ppm) separately or in combination, three times during 2020 and 2021 in a privet Farm located in a privet Farm located at West Mallawy Center-Minia Governorate. Applying glutathione was more effective than the royal jelly. Elevating two highest levels of all treatments did not significantly affected the studied parameters. However, the dual addition was more effective than the solo one and the best findings with regard to vegetative growth, leaf pigments and leaf chemical content were obtained when the vines sprayed with medium concentration royal jelly (200 ppm) and glutathione (500 ppm) thrice from economical point during two seasons.