This research involved 14-year-old Red Roomy grapevines. The experimental site was established at a private farm in West Matay Center, Minia Governorate, to investigate the effects of different concentrations of magnesium sulphate (0.05, 0.1, and 0.2%) and boric acid (0.025, 0.05, and 0.1%), along with their interactions, on the yield and the physical and chemical quality of Red Roomy grapevines during the 2023 and 2024 seasons. This study utilized a randomized complete block design comprising ten treatments with three duplicates. The findings indicated that the application of magnesium sulphate was more effective than boric acid, especially at a concentration of 0.2%, followed by 0.1%. The application of 0.1% boric acid combined with 0.2% magnesium sulphate proved to be the most effective treatment for the studied traits, closely followed by the combination of 0.05% boric acid and 0.1% magnesium sulphate, which showed no significant differences. The combination of middle concentrations was demonstrated the effectiveness economical in enhancing the yield, as well as the physical and chemical quality of Red Rooy grapevines under the Minia Governorate.