The present investigation was conducted during 2021 and 2022 on second and third ratoons of ‘Williams’ banana plants grown in a private farm situated at El- Gendia village, Beni-Mazar District El-Minia Governorate, Egypt, while the soil texture is loamy clay. Twelve stools, each one contains 3 plants uniforms in vigor of ‘Williams’ banana was chosen for achieving this study. The chosen stools are planted at 3 X 3 meters apart. The partial replaced of mineral nitrogen fertilizers with AMF inoculation (as a bio-fertilizer) at different percentages (25%, 50% and 75%) were examined. The results show that, replaced the mineral nitrogen fertilizer was lead to improving the plants mineral statues, yield (kg/plant) and fruit physic-chemical properties of ‘Williams’ banana fruit. It is clear that replaced 50% of mineral nitrogen fertilizer by using 50% of AMF inoculation produced the best mineral nutrients status, yield (kg), and fruit physicochemical properties of ‘Williams’ banana cultivar.