With an overall aims to study the effect of inoculation with Arbuscular Mmycorrhizal fungi (AMF) and phosphate dissolving bacteria “PDB” (Bacillus megatherium var. phosphaticum) on ‘Flame Seedless’ grapevines productivity and berries physicochemical properties grown under loamy clay soil at El-Minia Governorate conditions - Egypt. A field investigation included 10 treatments were achieved: Control (untreated vines), Inoculation with AMF at 50 g/vine, Inoculation with AMF at 100 g/vine, Inoculation with AMF at 150 g/vine, Inoculation with PDB at 50 ml/vine, Inoculation with PDB at 100 ml/vine, Inoculation with PDB at 150 ml/vine, Inoculation with AMF at 50 g/vine + 50 ml/vine PDB, Inoculation with AMF at 100 g/vine + 100 ml/vine PDB, Inoculation with AMF at 150 g/vine + 150 ml/vine PDB. The obtained results confirmed that Inoculation ‘Flame Seedless’ grapevines with PDB and AMF at different concentration cane significantly improved the productivity and berries quality. However, the combined inoculation with AMF + PDB shows more effective rather than using each one alone. Furthermore, the vines inoculated with AMF (at 150 g/vine) combined with PDF (at 150 ml/vine) present the best yield and berries physical and chemical properties.