This piece of work starts by describing the function structure and metabolism of lipids and also shows the importance of it, as start lipids are considered a heterogeneous group of molecules that have the common property of hydrophobicity. When we talk about lipids we may see a wide range
in structure from simple to complex hydrocarbon molecules, after that; go further to evaluate the production of lipolytic complexes that can be produced by various types of microorganisms isolated from different types of contaminated soils from many sources such as wastes surrounding factories,
slaughters and oily soils at the mechanic shops in order to select the best lipolytic microorganism which can survive in these soil conditions, to be used in biotechnological processes. We tested 14 types of
bacterial isolates taken from the previous places. Lipase production had been assayed in Enriched media, Selective media and Differential media with agar-based substrates. Then samples were incubated at 30
oC and 25 oC for 24 hrs. Growing showed 5 lipase producers.The optimization process was followed by culturing these bacteria in different conditions” temperature, pH” to select the one which have the
highest yield.Isolated strains were then going to the DNA gel electrophoresis process in order to confirm the presence of DNA, then the DNA is taken for a PCR to multiple the strand of DNA, after the DNA send for sequencing identified according to the 16S RNA and It revealed that these strains belong closely to bacillus cerus, pantonea sp., pseudomonas statceri andbacillus meaterium which show the highest
lipolytic activity.