This study was carried out mainly in Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Egypt, as well as in the laboratories of Agric. Microbiology Dept. at the Faculty of Agriculture, and Animal House at the Faculty of Veterinary Medicine, Zagazig University, Egypt. Adult male healthy Sprague-Dawley rats were used to study the effect of local lactic acid bacteria as a probiotics in animal feed. The important obtained results are the evolution of live body weight over 5 weeks in probioticsadministered groups witnessed relatively higher increases than in control. The obtained results revealed that the oral administration of probiotics gives the best results compared with supplemented of probiotics with diet. Probiotics treatments were associated with significant increases in the level of hemoglobin in rats. These findings
may indicate the absence of destruction of mature circulating cells or loss of cells from the circulation by haemorrhage, or reduced RBCs production, Moreover, without different changes were also observed in white blood corpuscles (WBC) in response to the administration of probiotics. The total concentration of serum cholesterol was significantly reduced from 136.2±3.8 mg/dl in the control to a mean value of 88.5±1.8 mg/dl in group treated with L. acidophilus oral administration. In contrast, The recorded values of renal parameter (urea
and creatinine) indicate significant reduction, for the all treats of probiotic with male rats, The levels of liver enzymes, i.e., aspartate aminotransferase, (AST), alanine aminotransferase (ALT) in rates receiving different probiotic treatments were significantly reduced by the probiotic application, the obtained reduction in results apparently refer to the absence of hepatotoxicity.