This work was carried out in Plant Tissue Culture Laboratory of Prof. Dr. Abd El-Fatah Helmy Belal, Faculty
of Environmental Agricultural Sciences, Arish University, North Sinai, Egypt during the period from 2016 to 2018.
This study was conducted to study the effect of medium type, explant source and growth regulator type and
concentration on micropropagation of E. citriodora plant which is grown in Sinai Peninsula. Results showed that
full strength of Murashige and Skoog's medium (MS) was the most suitable medium for seed germination and shoot
growth. Meanwhile, addition of BA at 1.00 mg l-1 was effective for improving shoot growth and development. Also,
multiplying of shoots was enhanced by using nodal cutting explants cultured on MS medium supplemented with
1.00 mg l-1 BA plus 1.00 mg l-1 NAA. On the other hand, shoot rooting was achieved by using MS medium
supplemented with 2.50 mg l-1
IBA. It is worth to mention that obtained plantlets were successfully acclimatized
(80% survivability) in a combination of peatmoss, vermiculite and washed sand or peatmoss and vermiculite at
equal volumes.