This experiment amid to study the effect of cultivars, growth regulator and number of sub-cultures on the
shoots formation of strawberry plantlets during multiplication stage and Random amplified polymorphic DNA
(RAPD) analysis of strawberry cultures in vitro during the fourth sub-culture. This experiment included 40
treatments, which were the combination between two strawberry cultivars (Festival and Sweet Charlie), five
treatments of growth regulator (BA and GA3) and four number of sub-cultures during shoots formation
(multiplication stage). The obtained results showed that, the maximum increment of growth measurement of
strawberry plantlets were recorded by Sweet Charlie cultivar .In addition , using ½ MS-medium without
supplemented with any growth regulators (BA and GA3) being the superior treatment for increasing both number of
leaves per shoot and shoot length. On the other hand, generally, the fourth sub-culture being the most effective
treatment on the growth measurement of strawberry plantlets during multiplication stage. Furthermore, Random
amplified polymorphic DNA (RAPD) analysis varied according to the two tested cultivars and the type of for
production of disease resistant plants and in plant breeding and crop improvement programs (Mohamed, 2003).