Biotechnological techniques are currently considered one of the most important means that have helped improve the production of various agricultural crops and the production of food and medicine. They introduce new methods to accelerate plant improvement and have enormous potential to overcome some obstacles that hinder the increase of agricultural production. In recent years, there has been growing interest in practical applications in the field of biotechnology, especially tissue culture technology, which relies on simple methods that do not require complex and costly laboratory equipment and can be practiced without significant investments in equipment and infrastructure. Plant tissue culture has emerged as one of the most prominent technologies of the twentieth century, pushing agricultural sciences beyond their traditional boundaries, as it has become possible to produce plants in the laboratory instead of the field, produce somatic embryos to be used instead of seeds by producing artificial seeds, and produce plant hybrids through protoplast fusion instead of crossbreeding and pollination.