The feasibility of applying the bio-stimulant substance Bio-Tech (NABTA-Bio) on growth, yield and quality of eight-year-old Red Globe and Crimson Seedless cv. grapevines grafted on Freedom rootstock was studied in this experiment in order to increase the add value for stirred yoghurt production for human health importance. This trial took place for the two successive seasons 2019 and 2020. Five treatments were applied whether as foliar spray or soil addition of Bio-Tech in different concentrations. Results revealed that vines treated with Bio-Tech (5L/ fed) (soil addition) + Bio-Tech (5L/ fed) (foliar spray) gave the highest values in all growth parameters and chemical characteristics of berries for both cultivars in addition to its positive effect when added to stirred yoghurt as a grape juice at two concentrations of 8 and 12 % through increasing the chemical, rheological and microbial properties of the yogurt during the storage period. Data showed a decrease in the acidity and microbial content and an increase in the total solids, protein and the content of phenolic substances and antioxidants when stored for 5 days with a reduction when stored for 10 days in the processed Crimson seedless more than in Red Globe cultivar. Also K, P, Mg and Fe % were increased in both cultivars varieties compared to the control, with superiority for Crimson seedless over Red Globe cv. Moreover, the stirred yoghurt made from Crimson seedless grape juice treated with Bio-Tech as bio-stimulant, whether at 8 or 12% had the highest sensory evaluation than the yogurt made from the untreated grapes.