One of the most prime winter-sown legume crops worldwide is the faba bean. It is benefit to enhance the yield of stable cultivars under proper environmental conditions, agricultural practices, and phosphorus fertilizer to increase faba bean production especially under climate change. The study was conducted in the two different locations, El-Kharga- and Al-Dakhla-New Valley during two successive seasons, 2019-2020 and 2020-2021, to study the effect of phosphorus fertilizer on the chemical composition of some faba bean cultivars at different location. The experiments included four broad bean cultivars i.e., Nubaria1, Sakha1, Mariout 2 and Wady1 and three phosphorus levels i.e., 0, 25and 50 kgP₂O₅\fed (12.5%P₂O₅). The results indicated that cultivar Sakha 1 produced the highest seed, straw and biological yield (kg\fed) and CHL.A, B. Fertilizing faba bean plants with 50 kg P₂O₅ produced the highest value of all studied traits during two seasons. The obtained results showed that the phosphorus fertilizers had an appositive effect on biochemical components in all cultivars at two locations. The treatment of (L2V2F2) has the highest chlorophyll a, b, and seed, straw and biological yield (kg\fed) while V4(Wady1) cultivar with F2, and L2 recorded the high values in carotenoids, proline, K, Ca, Mg, N, and total protein content in faba bean seeds about other cultivars. Seed yield (Kg/fed) was significantly positive correlation with chlorophyll Aand B, carotenes, nitrogen, protein (p<0.05). However, it was significantly strong positive correlation with proline, potassium, magnesium, and calcium with p<0.001.